A biomedical data science course using Python and available bioinformatics tools and techniques for the analysis of molecular biological data, including biosequences, microarrays, and networks. This course emphasizes practical skills rather than theory. Topics include advanced Python programming, R and Bioconductor, sequence alignment, MySQL database (DBI), web programming and services (CGI and django), genomics and proteomics data mining and analysis, machine learning, and methods for inferring and analyzing regulatory, protein-protein interaction, and metabolite networks.